November 10th, 2023
A new Method Unveiled: Creating Human-derived Protein-Based Microparticles featuring unique lamellar-like textures for Tissue Engineering
In the latest research published in Advanced Materials, researchers from COMPASS RG have introduced a direct, simple, and cost-effective method for creating protein-based round microparticles exhibiting unique textural surface features.
Ranging from 15 to 60 µm in diameter, these microparticles are sourced from human-derived platelet lysate (PL) protein, which carries a lower risk of triggering immune reactions than animal-derived materials.
Unlocking Nature’s Secret
PL proteins contain special amino acids, known as cysteines, which feature a thiol (-SH) side chain. However, these amino acids are typically found in their oxidative state as cystine (-S-S-, disulfide bonds), which plays a crucial role in the stability of protein tertiary structures. By cleverly manipulating the redox environment surrounding these proteins, we created non-natural disulfide bonds through intra- and inter-protein interactions, resulting in the formation of lamelar-like micro-units. Over time, these microunits self-assembled into complex microparticles with well-defined surface topographies.
A World of Potential
These PL-based microparticles demonstrated extraordinary cytocompatibility, which supported the formation of cell-guided microtissues. Early studies also indicated that the surface topography of these microparticles can promote osteogenic behaviour, making them potentially useful for bone regeneration. Additionally, these PL microparticles can be used as injectable systems for cell supports.
As we enter a new era in biomaterials and tissue engineering/regeneration, these human-derived protein-based microparticles offer a glimpse into an exciting future.
Check out and explore the world of PL microparticles on the pursuit of revolutionizing biotechnology and science advancements: https://doi.org/10.1002/adma.202304659